Early-life matters: The role of fetal adrenal steroids in the relationship between cytokines within the placental circulation and cognitive development among infants in the Philippines.

Prenatal exposure to inflammation is related to the risk for cognitive impairment in offspring. However, mechanisms underlying the link between inflammatory cytokines at the maternal-fetal interface and human cognitive development are largely unknown. This study addressed this research gap by examining whether i) cytokines within the placenta are associated with different domains of neurocognitive development during infancy, and ii) if DHEA-S in cord blood mediates these associations. We also explored the role of early-life socioeconomic status (SES) in moderating the effect of fetal adrenal steroids on cognitive development in low- and middle-income country contexts. A cohort of 242 mother-infant dyads in Leyte, the Philippines participated in the study and all of them were followed from early pregnancy until 12-months. Concentrations of pro- and anti-inflammatory cytokines in the placenta, and DHEA-S in cord blood collected at delivery were evaluated. The multifactorial aspects of the infant's cognitive functioning were assessed based on the Bayley Scales of Infant Development, third edition (BSID-III). We used Structural Equation Modelling (SEM) with an orthogonal rotation to examine associated paths among latent variables of pro- and anti-inflammatory cytokines in the placenta, fetal neuroendocrine factors, and cognitive development. Pathway analyses showed that both pro- and anti-inflammatory cytokines in the placenta were indirectly related to cognitive (p < 0.05) and language developmental outcomes (p < 0.1) via DHEA-S in cord blood among the low SES group. Yet, we found no statistically significant indirect effect of pro- or anti-inflammatory cytokines on neurocognitive development among the high SES sub-sample. This study extends our understanding of how early-life socioeconomic conditions modify biological pathways underlying the relationship between prenatal factors and postpartum cognitive development.

Prenylated Chrysin Derivatives as Partial PPARγ Agonists with Adiponectin Secretion-Inducing Activity.

Decreased circulating adiponectin levels are associated with an increased risk of human metabolic diseases. The chemical-mediated upregulation of adiponectin biosynthesis has been proposed as a novel therapeutic approach to managing hypoadiponectinemia-associated diseases. In preliminary screening, the natural flavonoid chrysin (1) exhibited adiponectin secretion-inducing activity during adipogenesis in human bone marrow mesenchymal stem cells (hBM-MSCs). Here, we provide the 7-prenylated chrysin derivatives, chrysin 5-benzyl-7-prenylether compound 10 and chrysin 5,7-diprenylether compound 11, with the improved pharmacological profile compared with chrysin (1). Nuclear receptor binding and ligand-induced coactivator recruitment assays revealed that compounds 10 and 11 functioned as peroxisome proliferator-activated receptor (PPAR)γ partial agonists. These findings were supported by molecular docking simulation, followed by experimental validation. Notably, compound 11 showed PPARγ binding affinity as potent as that of the PPARγ agonists pioglitazone and telmisartan. This study presents a novel PPARγ partial agonist pharmacophore and suggests that prenylated chrysin derivatives have therapeutic potential in various human diseases associated with hypoadiponectinemia.

Social Participation Through the Retirement Transition: Differences by Gender and Employment Status.

This study examines how retirees' formal and informal social participation change over time and investigates gendered differences. Seven waves of the Korean Longitudinal Study of Ageing track the frequency of social participation. A comparison group of employed individuals, weighted with coarsened exact matching, controls for age and time trends, and mixed model regressions estimate changes over time. Retirees show a gradual decline in the frequency of meeting friends and an abrupt decrease in the frequency of attending a social gathering, compared to their working peers. These trends are much stronger for men than women, and compound pre-existing gender differences in social participation. The more severe decline in the social participation of men is of great concern and points to the persistence of gendered employment structures into the retirement transition, putting Korean retirees at risk of social isolation and related health deterioration.

No travel worsens depression: reciprocal relationship between travel and depression among older adults.

BACKGROUND: The aim of this study was to examine the bidirectional relationship between depression and travel. METHOD: We analyzed 8524 participants' data obtained from the 2008 to 2016 waves of the Korean Longitudinal Study of Ageing, a prospective cohort study. Depression was diagnosed using the Center for Epidemiological Studies Depression Scale: 10-Items (CES-D10), with scores of 4 or higher indicating depression. We used a generalized estimating equation and a cross-lagged panel model for statistical analysis. RESULTS: Participants who had not traveled for one year had a 71% higher risk of suffering from depression in the following year than did those who had traveled [relative risk (RR) = 1.71, P < 0.001], and participants with depression had more than double the increased risk of not traveling than did those not currently suffering from depression (RR = 2.08, P < 0.001). The cross-lagged panel model confirmed the vicious cycle involving the amount of travel and score on the CES-D10; individuals who traveled more frequently were more likely to have lower scores on the CES-D10 (coefficient = - 0.04 to - 0.03, Ps < 0.01), and individuals with higher scores were less likely to travel (coefficient = - 0.06 to - 0.03, Ps < 0.01). CONCLUSIONS: The risk of depression increases for people who do not travel, and a reciprocal relationship exists between travel and depression.

Nyuzenamide C, an Antiangiogenic Epoxy Cinnamic Acid-Containing Bicyclic Peptide from a Riverine Streptomyces sp.

A new nonribosomal peptide, nyuzenamide C (1), was discovered from riverine sediment-derived Streptomyces sp. DM14. Comprehensive analysis of the spectroscopic data of nyuzenamide C (1) revealed that 1 has a bicyclic backbone composed of six common amino acid residues (Asn, Leu, Pro, Gly, Val, and Thr) and four nonproteinogenic amino acid units, including hydroxyglycine, ß-hydroxyphenylalanine, p-hydroxyphenylglycine, and 3,ß-dihydroxytyrosine, along with 1,2-epoxypropyl cinnamic acid. The absolute configuration of 1 was proposed by J-based configuration analysis, the advanced Marfey's method, quantum mechanics-based DP4 calculations, and bioinformatic analysis of its nonribosomal peptide synthetase biosynthetic gene cluster. Nyuzenamide C (1) displayed antiangiogenic activity in human umbilical vein endothelial cells and induced quinone reductase in murine Hepa-1c1c7 cells.

Cohort matters: The relationships between living arrangements and psychological health from the Korean Longitudinal Study of Aging (KLoSA).

BACKGROUND: Little is known about cohort variations in the relationships between living arrangements and psychological health among older adults. The current study evaluated whether cohort differences in the intergenerational support affect the differences in the mental health benefits of multigenerational living arrangements, and how they do so. METHODS: Using panel regression models with lagged variables based on South Korean data, we compared the shape of the relationships between living arrangements and psychological conditions of two cohorts of older adults. RESULTS: The study found birth cohort differences in older adults' psychological well-being. Among older adults in recent cohorts, living in a multigenerational household was positively associated with depression and negatively linked with life satisfaction. The cohort variations were partly explained by children's marital status and home ownership. CONCLUSION: The birth cohort variations in psychological health implications of living arrangements may reflect the changing patterns of intergenerational support and family values.

Evaluation of Two Methods (Inside-Out/Outside-In) Inferior Articular Process Resection for Uniportal Full Endoscopic Posterolateral Transforaminal Lumbar Interbody Fusion: Technical Note.

Objective: There is limited literature comparing the uniportal full endoscopic posterolateral transforaminal lumbar interbody fusion outside-in approach (ETLIF (O)) with the inside-out approach (ETLIF (I)). Methods: Radiological evaluation was performed on disc height restoration and coronal wedging angle, and operation time (inferior articular process resection time/total operation time) and clinical evaluation were made. Result: 48 cases of inside-out and 38 cases of outside-in cases were included. Compared to inside-out, the outside-in approach had significantly less operative time required to resect inferior articular process: 36.55 ± 10.37, and total operative time: 87.45 ± 20.14 min compared to 49.83 ± 23.97 and 102.56 ± 36.53 min, respectively, for the inside-out approach, p < 0.05. Compared to the preoperative state, both cohorts achieved significant improvement of VAS and ODI at post-operative 1 week, 3 months and at final follow up. Both cohorts achieved statistically significant increased disc height with 5.00 ± 2.87 mm, 5.49 ± 2.33 mm and statistically significant improvement in coronal wedge angle with 1.76 ± 1.63°, 3.24 ± 2.92° in the inside-out and outside-in approaches respectively. Conclusions: Complete removal of inferior articular process is the key part of endoscopic fusion with two methods that can be applied: an inside-out approach or an outside-in approach. Comparing both techniques, the outside-in approach has a shorter operative time required for inferior articular process resection and total length of operation with similar good clinical and radiological outcomes.

The Country That Never Retires: The Gendered Pathways to Retirement in South Korea.

OBJECTIVES: Among all Organization for Economic Cooperation and Development countries, South Korean older adults work until the latest age. We investigate the extent to which work experiences over the life course and family circumstances can be associated with older workers' incentives to remain in the labor force beyond the statutory pension age. We explore gender-specific patterns of labor force exit and labor force re-entry in later life. METHODS: Using panel data of South Korean older workers and retirees from 2006 to 2016, we estimate multilevel discrete-time models with random effects to predict their labor force transition process that unfolds over time. RESULTS: Results show that skilled manual workers are less likely to exit employment and more likely to re-enter the labor force. A longer history of self-employment is related to later retirement. The relationship between career characteristics and the risk of retirement is only significant for men. Late-aged employment transition among women appears to be more related to family conditions. Women who receive financial support from adult offspring are more likely to remain out of the labor force but this relationship is not pronounced among men. DISCUSSION: Policies aimed at extending working lives need to provide various types of social support to older job seekers, especially those who had low-class jobs and those without family networks.

The Comparative Macro-Level Ageism Index: An International Comparison.

Ageism, socially constructed discrimination against seniors, influences the quality of life of older adults. This study aimed to compare ageism in 15 Organization for Economic Cooperation and Development (OECD) countries using the scientifically developed Comparative Macro-Level Ageism Index (CMAI). We developed the CMAI by reviewing previous index research on the life of older people and conducting expert interviews using a Delphi method. We identified five domains, economic status, health, employment, environment, and social participation, and 17 indicators of ageism. Standardized ageism scores were compared across 15 OECD countries. The results indicated that the overall ageism score was highest in Turkey and lowest in Japan. Turkey was the most likely while South Korea was the least likely to practice age discrimination in the workplace. Yet, South Korea had the highest score for discriminating against seniors based on economic status while Spain had the lowest score in this domain. Japan had favorable conditions for economic status, health status, and social participation for older adults. Implications of this study and suggestions for further studies on developing an objective indicator of structural age discrimination are discussed.

Global Seasonality of Human Coronaviruses: A Systematic Review.

In the context of the coronavirus disease 2019 pandemic, we aimed to systematically address the global seasonal patterns of human coronavirus (HCoV) infections. We identified relevant articles from MEDLINE, EMBASE, and CINAHL Plus as of May 11, 2020. The main outcomes were the peak months of HCoV infections each year and the months during which more than 5% of positive respiratory specimen tests were attributable to HCoV. Of 707 articles reviewed, 22 met the inclusion criteria. The annual percentage of HCoV infections reached a peak in February globally. We found a higher HCoV positivity rate among studies that tested only children (median: 5.9%, range: 0.9%-18.4%), compared with other studies of adults alone (median: 5.2%, range: 3.3%-7.1%) or the entire population (median: 1.9%, range: 0.2%-8.1%). We found the largest global peak of HCoV during the winter season, with the highest rate of positivity among children.

Mortality and suicide in schizophrenia: 21-year follow-up in rural China.

BACKGROUND: Little is known about the trend and predictors of 21-year mortality and suicide patterns in persons with schizophrenia. AIMS: To explore the trend and predictors of 21-year mortality and suicide in persons with schizophrenia in rural China. METHOD: This longitudinal follow-up study included 510 persons with schizophrenia who were identified in a mental health survey of individuals (≥15 years old) in 1994 in six townships of Xinjin County, Chengdu, China, and followed up in three waves until 2015. Kaplan-Meier survival analysis and Cox hazard regressions were conducted. RESULTS: Of the 510 participants, 196 died (38.4% mortality) between 1994 and 2015; 13.8% of the deaths (n = 27) were due to suicide. Life expectancy was lower for men than for women (50.6 v. 58.5 years). Males consistently showed higher rates of mortality and suicide than females. Older participants had higher mortality (hazard ratio HR = 1.03, 95% CI 1.01-1.05) but lower suicide rates (HR = 0.95, 95% CI 0.93-0.98) than their younger counterparts. Poor family attitudes were associated with all-cause mortality and death due to other causes; no previous hospital admission and a history of suicide attempts independently predicted death by suicide. CONCLUSIONS: Our findings suggest there is a high mortality and suicide rate in persons with schizophrenia in rural China, with different predictive factors for mortality and suicide. It is important to develop culture-specific, demographically tailored and community-based mental healthcare and to strengthen family intervention to improve the long-term outcome of persons with schizophrenia.

Associations among working hours, sleep duration, self-rated health, and health-related quality of life in Korean men.

BACKGROUND: This study aimed to examine the relationship between working hours and health-related quality of life (HRQOL) in men and to determine whether this relationship was mediated by sleep duration and self-rated health (SRH). MATERIALS AND METHODS: Our study population included 2141 working men aged 20 to 49 years old from the Korea National Health and Nutrition Examination Survey 2016-2017. Working hours, SRH, and HRQOL were measured by a structured questionnaire survey. Sleep duration was calculated based on self-reported data. We investigated the association between working hours and HRQOL and performed a mediation analysis to evaluate the contributions of sleep duration and poor SRH to this relationship. RESULTS: This study identified a significant association between working hours and HRQOL. Long working hours directly and indirectly affected HRQOL through poor SRH. Men who overworked were more likely to report poor HRQOL due to worsened SRH. Poor SRH was responsible for 26.3% of this relationship. But sleep duration did not explain the relationship between working hours and HRQOL. CONCLUSIONS: Working hours were directly associated with HRQOL in men. Furthermore, there was an indirect effect of working hours on HRQOL mediated by poorer SRH. An improved work-life balance is necessary to promote men's health and quality of life.

Assembly and Folding Properties of Cytosolic IgG Intrabodies.

Intrabodies, antibodies expressed within cells, offer an interesting way to target intracellular molecules, making them potentially useful for biotechnology and medicine. However, it remains controversial whether full-size IgG intrabodies expressed in the reducing environment of the cytosol of mammalian cells are workable and structurally sound. Herein, we settle this issue with a systematic investigation of the structure and functionality of four chimeric IgG1s with distinct variable (V) domains but identical constant (C) domains. Full-size IgGs expressed in the cytosol of HEK293 cells were either assembly-competent or -incompetent, depending on the intrinsic properties of the V regions. Structural integrity of the C region is required for H:L association and the formation of a functional antigen-binding site. Partial intrachain disulfide bond formation occurs in both H and L chains of cytosolic IgG intrabodies, whereas interchain disulfide bond formation was absent and dispensable for functional assembly. IgG1s expressed in the cytosol and via the ER were shown to assemble differently. Our findings provide insight into the features and possible utilization of full-size IgGs as cytosolic antibodies in biotechnological and medical applications.

Gender matters: Productive social engagement and the subsequent cognitive changes among older adults.

This study examines the relationships between productive social engagement and cognitive functioning trajectories of older adults in South Korea and how the nature of the relationships differs for men and women. We exploit data from a nationally representative longitudinal survey of South Korea from 2006 to 2014 and apply the Growth Mixture Modeling approach to disentangle health causation from health selection processes. We find that socially productive activities are associated with more favorable cognitive functioning trajectories, independent of an individual's baseline health and cognitive status. The odds of maintaining higher cognitive functioning are greater for men who had participated in socially productive activities on a monthly basis. Social engagement protects against rapid cognitive decline for women, particularly for those with lower education. Among men, social activities in friendship/alumni organizations are likely to help maintain good cognitive functioning in older age while for women with lower cognition, religious activities may protect against cognitive decline.

Adult children's educational attainment and the cognitive trajectories of older parents in South Korea.

Little is known about whether and how adult children's educational resources are associated with aging parents' cognitive changes over time. Using a nationally representative survey from the Korean Longitudinal Study of Aging (2006-2014), the current study examined three related questions: whether adult children's education is independently associated with parents' latent trajectory classes of cognitive functioning; whether this association is explained by behavioral, psychological, and financial pathways; and whether the association varies by the gender of the child. Adult children's education is negatively associated with the unfavorable cognitive functioning trajectories of parents. Children's education predicts parents' higher perceived life satisfaction and upward financial transfers, which are some of the pathways through which children's education appears to prevent subsequent cognitive impairment in parents. Sons' education matters more for preventing the onset of dementia, and daughters' educational resources are linked to the prevention of cognitive decline among parents.

Heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) function as endocytic receptors for an internalizing anti-nucleic acid antibody.

A subset of monoclonal anti-DNA autoantibodies enters a variety of living cells. Here, we aimed to identify the endocytic receptors recognized by an internalizing anti-nucleic acid autoantibody, the 3D8 single-chain variable fragment (scFv). We found that cell surface binding and internalization of 3D8 scFv were inhibited markedly in soluble heparan sulfate (HS)/chondroitin sulfate (CS)-deficient or -removed cells and in the presence of soluble HS and CS. 3D8 scFv colocalized intracellularly with either HS proteoglycans (HSPGs) or CSPGs in HeLa cells. 3D8 scFv was co-endocytosed and co-precipitated with representative individual HSPG and CSPG molecules: syndecan-2 (a transmembrane HSPG), glypican-3 (a glycosylphosphatidylinositol (GPI)-anchored HSPG); CD44 (a transmembrane CSPG); and brevican (a GPI-anchored CSPG). Collected data indicate that 3D8 scFv binds to the negatively charged sugar chains of both HSPGs and CSPGs and is then internalized along with these molecules, irrespective of how these proteoglycans are associated with the cell membrane. This is the first study to show that anti-DNA antibodies enter cells via both HSPGs and CSPGs simultaneously. The data may aid understanding of endocytic receptors that bind anti-DNA autoantibodies. The study also provides insight into potential cell membrane targets for macromolecular delivery.

Slender women and overweight men: gender differences in the educational gradient in body weight in South Korea.

BACKGROUND: Little is known about the gender-specific mechanisms through which education is associated with weight status in societies that have experienced a rapid rise in their obesity rates. This study extends previous literature by examining how the link between education and weight status operates within the structure of gender relations in South Korea where huge gender differences have been observed in the educational inequalities in weight status. METHODS: Using the Korean National Health Survey (N = 17,947) conducted in 2008-2012 conditional quantile regression models were estimated to assess the associations between education and body weight distribution. The mean difference in the predicted probabilities of perceiving body image as average was compared by educational attainment for women and men while setting all other covariates at their means. RESULTS: Highly educated women were more likely to utilize their human capital to obtain slender body shape and the relationship was not mediated by economic resources. In contrast, education was positively associated with being overweight and obesity among men, for whom behaviors promoting healthy weight often conflict with a collective ideology at work that strongly supports long work hours and heavy alcohol consumption. Furthermore, Korean men were more likely to under-perceive their body size than Korean women, that is, overweight men tend to consider themselves to be of 'average' weight, regardless of their educational attainment. CONCLUSIONS: Current study found that gender inequalities in social status in South Korea operate to affect the relationship between education and weight status among men and women in unique ways. Weight status can be socially patterned by the interplay between education, economic, and behavioral resources within the structure of gender relations.

The catalytic activity of a recombinant single chain variable fragment nucleic acid-hydrolysing antibody varies with fusion tag and expression host.

The antigen-binding properties of single chain Fv antibodies (scFvs) can vary depending on the position and type of fusion tag used, as well as the host cells used for expression. The issue is even more complicated with a catalytic scFv antibody that binds and hydrolyses a specific antigen. Herein, we investigated the antigen-binding and -hydrolysing activities of the catalytic anti-nucleic acid antibody 3D8 scFv expressed in Escherichia coli or HEK293f cells with or without additional amino acid residues at the N- and C-termini. DNA-binding activity was retained in all recombinant forms. However, the DNA-hydrolysing activity varied drastically between forms. The DNA-hydrolysing activity of E. coli-derived 3D8 scFvs was not affected by the presence of a C-terminal human influenza haemagglutinin (HA) or His tag. By contrast, the activity of HEK293f-derived 3D8 scFvs was completely lost when additional residues were included at the N-terminus and/or when a His tag was incorporated at the C-terminus, whereas a HA tag at the C-terminus did not diminish activity. Thus, we demonstrate that the antigen-binding and catalytic activities of a catalytic antibody can be separately affected by the presence of additional residues at the N- and C-termini, and by the host cell type.

Early-Life Socioeconomic Conditions, Height, and Functional Health Status in Later Life: Evidence From South Korea.

The article contributes to the literature on health inequalities by examining the associations between early-life socioeconomic status (SES), height, and functional limitations in later life among Korean men and women. The findings were based on 1562 men and 1801 women aged 50 to 79 years who participated in the 2005 Korean Labor and Income Panel Study (KLIPS). Among men, 17.8% reported having functional limitations, compared to 31.8% among women. The ordered logistic regression analyses showed that for Korean men, the relative contribution of early-life SES to functional health was less important than that of adult SES. For Korean women, childhood SES was a significant predictor of functional limitations after controlling for attained SES. Short stature was associated with poorer functional health only among men and the relationship was explained by adult characteristics. The results imply that long-term effect of childhood deprivation on health may be stronger among women in Korean society.